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Silk protein is a natural protein derived from yeast, with various biological activities and medical values, and has become one of the prospects for the new generation of biomedical materials. This article will provide a detailed introduction to the production process of brushed protein.
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1. Raw material procurement
The main raw materials for the production of silk protein are yeast fungal bodies, sugars, nitrogen sources, etc. These substances must be purchased in a high-purity environment to ensure the purity and quality of silk protein. At the same time, it is necessary to prepare the necessary air, water, and electricity for the preparation of silk protein.
2. Preprocessing
The purchased raw materials need to undergo multiple pre-treatment steps, including cleaning, crushing, steaming, etc., to ultimately obtain approximately pure yeast cells. In addition, it is necessary to treat raw materials such as sugar and nitrogen sources to ensure sufficient supply of nutrients during the subsequent fermentation process.
3. Fermentation
Fermentation is the core process of silk protein production. By mixing yeast cells with a culture medium containing various nutrients and utilizing the metabolic capacity of yeast, the silk protein secreted is synthesized and self secreted into the culture medium. During the fermentation process, strict control of temperature, pH value, aeration rate, stirring and other conditions is required to ensure the yield and purity of silk protein.
4. Separation and purification
The culture medium produced by fermentation contains a large amount of yeast cells and other components, which require separation and purification to obtain silk protein. The separation and purification process includes centrifugation, ultrafiltration, ion exchange, gel filtration and other links. Through these processing steps, the influence of yeast components in the culture medium can be gradually weakened, while improving the purity and stability of the silk protein.
5. Concentrated drying
In the process of separation and purification, it is necessary to further concentrate the obtained silk protein to reduce its volume and improve its purity. The process of concentration and drying needs to be carried out at low temperatures to avoid the denaturation and degradation of silk protein. In addition, attention should be paid to avoiding excessive storage and transportation to ensure the quality and stability of the brushed protein.
Overall, the production process of brushed protein includes multiple steps such as raw material procurement, pre-treatment, fermentation, separation and purification, concentration and drying, each of which has its own unique technical requirements and precautions. Only by strictly controlling the process conditions and quality standards of each link can high-quality brushed protein products be ultimately obtained.
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